GMT Tutorial Exercise 26 - Workflow 1 - Step 1

Generate the raw Luminex data with the BioPlex Manager^TM Software

1. Perform a run with the BioPlex Manager^TM software. After a reading is complete, a results file is generated and automatically opens.




2. Click on the Raw Data button (in the left-hand side panel of the results file view) to open the Raw Data window of the results file.




3. Click on the Show/Hide Bead Statistics Columns button on the table toolbar, so that the number of beads collected for each analyte will be displayed in parentheses after the analyte fluorescent intensity in the exported file.




4. Choose File » Export to Excel from the file menu. A new Excel file containing the raw data is generated.




5. Save the Excel file with raw data to your computer.
   Below is an example of how the exported data will look. Note that bead numbers are provided in parentheses behind mean reporter dye intensities: