GMT Tutorial Exercise 23 - Workflow 2 - Step 14

Separate PCR products on the ABI3730

• Forward the PCR products to your DNA fragment separation service provider, together with the Sample Submission File generated by the macro as descried previously in this workflow.
• If using an ABI3730, fragment separation results will be returned as an electronic folder with an *.fsa file for each of the sample wells that were analysed.
• Beside the actual electrotrace, each of the *.fsa files also contains information from the Sample Submission File, e.g. the sample name, the name of the marker panel and the size standard used in a well. This information can be accessed when opening *.fsa files in GeneMapper, if it is set up according to the instructions in this workflow.
• Proceed to the next step of the workflow for instructions how to analyse the *.fsa files in GeneMapper.