GMT Tutorial Exercise 22 - Workflow 1 - Step 8

Using the macro - the "Primer Dilution Plate Layout" worksheet

• The Primer Dilution Plate Layout worksheet of the macro shows how dilutions of the "locus-specific" primers should be pipetted on a 96-well plate. Preparing the primers in this way will simplify mixing "locus-specific" primers and PCR master mixes for the different DNA samples by manual or robotic multichannel pipetting, while ensuring that "marker panels" are not mixed up.
• Click on Print Preview to print a copy for use during PCR set-up.
• Click on Next Sheet to begin the [next step] of the workflow.